Architecture & Interface Background

Hardware-Software Interfaces

The Ethernet/PCIe DAQ board (EDAQ) has multiple hardware-software interface options to provide system architecture flexibility for the integrator.

Two distinct software interfaces between the EDAQ hardware and the host PC software are utilized, generally referred to as the Control interface and the Capture interface:

Control Interface

The Control interface is low-bandwidth, bidirectional, and consists of command & control, diagnostics, configuration, and status messaging between the EDAQ hardware and host software. It is also used for updating firmware on the EDAQ board. The Control Interface is also used for command & control messaging with the Laser Engine board and Camera Link DAQ board.

Capture Interface

The Capture interface is high-bandwidth, unidirectional* (from EDAQ to host only), and consists of the OCT image data stream generated by the EDAQ and transmitted to the host software for subsequent display, analysis, and archival by the user. The Capture Interface is relevant to the Ethernet/PCIe DAQ only (not the Laser Engine or Camera Link DAQ).

* NOTE: the Capture interface does also provide some low-bandwidth bidirectional control functionality (i.e. register R/W) for DAQs connected via PCIe.

Physical Interfaces

There are also several physical interfaces supported between the EDAQ hardware and host PC, including USB 2.0, Gigabit Ethernet, USB 3.0 [1], and PCI-Express (PCIe). For the Axsun Integrated Engine configuration, a RS-232 interface directly between the EDAQ and the Laser Engine board provides a convenient pass-thru of laser messages via this serial connection when the EDAQ is connected to the host via Ethernet/USB 3.0.

The Control and Capture software interfaces are supported on the EDAQ’s physical interfaces according to the following matrix:

	USB 2.0	Gigabit Ethernet (USB 3.0 via dongle)	PCIe
Capture Interface (image stream from DAQ)	NO	YES	YES
Capture Interface (DAQ commands & status)	NO	NO	YES (Register R/W)
Control Interface (DAQ commands & status)	YES	YES	NO
Control Interface (to Laser via DAQ pass-thru)	NO (need USB to Laser)	YES	NO

[1] All Ethernet functionality described in this manual is also supported over USB 3.0 via a separate Ethernet-to-USB 3.0 converter based on the Realtek RTL8153 chipset. For example: https://www.amazon.com/Anker-Portable-Aluminum-Ethernet-Supporting/dp/B00PC0H9IE

Software Libraries

The Capture and Control software interfaces are decomposed into separate Windows OS dynamic link libraries: AxsunOCTControl.dll , AxsunOCTControl_LW.dll and AxsunOCTCapture.dll (or *.so for corresponding Linux OS shared object or *.dylib for corresponding MacOS shared object).

Axsun provides basic GUI tools for exercising the functionality provided in the Capture and Control libraries, as well as APIs and sample code for each of the libraries for system integrators to develop custom client applications based on these libraries.

This multi-library software architecture is convenient in the API integration phase of custom software development because it allows the integrator to debug new code based on one library while relying on an Axsun-provided GUI tool to exercise functionality in the other library. Once new code has been developed at the module level for both libraries separately, final integration of these modules into a single application can be achieved with more confidence and less debugging complexity.

A summary of the Capture and Control library characteristics is provided in the following table:

	AxsunOCTCapture	AxsunOCTControl	AxsunOCTControl_LW (light-weight version)
Primary Functionality	Capture image stream from EDAQ, enqueue in host RAM, return to user on request for display or archival, PCIe imaging control	Control DAQ imaging mode and settings, control laser emission on/off	Control DAQ imaging mode and settings, control laser emission on/off
Secondary Functionality	OpenGL rendering of images including polar scan conversion (Windows only), save/load images to disk, galvo scanner waveform programming	Update firmware, perform diagnostics, control optional motorized variable delay line (VDL)	Control optional motorized variable delay line (VDL)
3rd-Party Dependencies (free, open-source)	pcap (Ethernet only), TurboJPEG, OpenCV (optional), PCIe Device Drivers (PCIe only)	Microsoft .NET, LibUSBDotNet	libusb
Library Interface	ANSI C	Managed C# (or C++ via COM)	ANSI C
OS Support	Windows 10/11, Linux	Windows 7 or 10/11	Windows 10/11, Linux
Axsun GUI Tools	Image Capture Tool	OCT Host or Hardware Control Tool***	Hardware Control Tool***
Sample Code Available	LabVIEW, C/C++	LabVIEW, C#, C++	LabVIEW, C/C++
Supported Physical Interfaces	Ethernet / USB 3.0, PCIe	Ethernet / USB 3.0, USB 2.0, RS-232 (to laser)	Ethernet / USB 3.0, USB 2.0, RS-232 (to laser)
Architecture	64-bit x86	32- or 64-bit x86	64-bit x86
Library Name	AxsunOCTCapture (.dll, .so)	AxsunOCTControl.dll	AxsunOCTControl_LW (.dll, .so)

*** WARNING: Do not open multiple instances of AxsunOCTControl or AxsunOCTControl_LW at the same time, either via the Axsun-supplied GUI tools or via custom applications interfaced with these libraries.

Hardware Architecture

The Axsun EDAQ employs both a small microcontroller and a large FPGA processor. The microcontroller is responsible for managing bidirectional communications with the host PC via the AxsunOCTControl/Control_LW interface and with a connected Laser Engine board, for loading its own program and the FPGA program at power-on, for managing non-volatile configuration settings, and for providing USB 2.0 I/O. The FPGA is responsible for computationally intensive tasks such as OCT image formation (Fourier transformation, compression, etc.) and also provides the Gigabit Ethernet and PCIe I/O.

The integration strategy for systems based on the EDAQ will likely depend on whether Ethernet or PCIe is the preferred method for transmitting high-bandwidth image data for the specific application. The Ethernet-based implementation is more straightforward from the perspective of cable interconnects and is easier to achieve a high degree of portability, especially if physical separation between the DAQ and the host PC is required and if JPEG image compression is tolerable. The PCIe-based implementation requires close proximity between the DAQ and the host PC (unless optical Thunderbolt cables are used), but supports data transfer of raw, partially-processed, or fully-processed data without lossy compression at full A-line rates.

The following block diagrams show basic Ethernet, USB, and PCIe interconnections for the EDAQ and associated Laser Engine board in the Axsun Integrated Engine, along with the software libraries which utilize these interfaces:

Ethernet Mode (also USB 3.0 Mode)

Connections to Host PC via Ethernet.

• For a PC with USB 3.0 but without RJ-45 connectivity and a built-in network adapter, use a GigE-to-USB 3.0 converter dongle instead.

PCIe Mode

Connections to Host PC via PCIe (data) and USB 2.0 (command messages)

GUI Tools

The AxsunOCTCapture and AxsunOCTControl/Control_LW APIs enable users to integrate library functionality in custom executables for application-specific workflows and requirements. Axsun also provides several Windows-based GUI Tools for exercising basic library functionality without the need for custom software development.

NOTE: The GUI Tools provided by Axsun are not intended to be fully-featured or to support complex workflows for all OCT imaging applications, but are helpful for engineering tasks such as:

• optical integration and OCT image optimization,

• understanding the features and capabilities of the Axsun hardware,

• exercising API functions using simple button presses,

• verifying successful installation of software dependencies,

• debugging during custom software development,

• configuration of power-on defaults, and

• firmware upgrades, diagnostics, or other field service maintenance.

NOTE: Representations and descriptions of the user interfaces shown in this reference manual and other online documentation may not match precisely with the specific GUI Tool version installed on your system. However, the basic functionality available through these tools is consistent across all versions.

NOTE: LabVIEW-based GUI Tools are displayed correctly when the Windows text size is set at 100%, otherwise button labels and other text may not be properly rendered on all systems:

Image Capture Tool

The Image Capture Tool (AxsunImageCaptureTool.exe) or "ICT" is a LabVIEW-based application which exemplifies the core functionality provided by the AxsunOCTCapture library, including live image display, on-demand image retrieval by unique image number, indication of the EDAQ’s imaging mode, Image_sync trigger status, and main image buffer statistics.

Secondary functionality includes controls for the direct OpenGL rendering features, real-time image averaging, image cropping, saving & loading, and some FPGA register access (PCIe interface only).

The Axsun Image Capture Tool (ICT)

Image Capture Tool

Hardware Control Tool

The Hardware Control Tool (AxsunHardwareControlTool.exe) or "HWCT" is a LabVIEW-based application that provides push-button operation for many of the basic functions of the EDAQ and laser via the AxsunOCTControl/Control_LW libraries, including the indication of hardware connectivity, toggling laser emission on/off, toggling Ethernet DAQ imaging mode on/off, starting a burst record operation, adjusting the K-clock delay, and controlling a motorized variable delay line or aiming laser (if installed).

High-level DAQ functionality (achieved via scripted low-level FPGA register writes) is provided on separate tabs and includes setting power-on defaults, programming a user-defined windowing function, polarization channel selection, dispersion compensation, background subtraction, and raw data / bypass mode configuration.

The Axsun Hardware Control Tool (HWCT)

Hardware Control Tool

OCT Host

OCT Host (OCTHost.exe) is a Windows Forms-based GUI that provides a subset of the features of the Hardware Control Tool (also via the AxsunOCTControl library), including toggling laser emission on/off, indicating hardware connectivity state, adjusting the K-clock delay, and controlling a motorized variable delay line (if installed). OCT Host also provides more advanced laser diagnostics, maintenance, and debugging information, as well as system firmware upgrade capabilities. EDAQ functionally in OCT Host is limited to low-level operations such as FPGA register reading/writing and setting power-on defaults in non-volatile RAM.

WARNING: The OCT Host and Hardware Control Tool applications must not both be running concurrently; the EDAQ microprocessor will not support Ethernet communication with multiple instances of the AxsunOCTControl/Control_LW library at the same time. If multiple instances are accidentally established, a full application and EDAQ hardware restart is recommended for deterministic communication.

Axsun OCT Host GUI

OCT Host

Summary of GUI Tools

The following table summarizes some of the characteristics and functionality of the Axsun-provided GUI Tools:

	OCT Host	Hardware Control Tool	Image Capture Tool
Axsun API Library Dependency	AxsunOCTControl	AxsunOCTControl or AxsunOCTControl_LW	AxsunOCTCapture
Other Dependencies	MS .NET	LabVIEW Run-Time	LabVIEW Run-Time
Physical Interfaces	USB 2.0, Ethernet, RS-232	USB 2.0, Ethernet, RS-232	Ethernet, PCIe
Capture & Display OCT Images	-	-	YES
Save & Load Captured Images	-	-	YES
Control & Query Laser Emission	YES	YES	-
Adjust K-clock Delay	YES	YES	-
Update Firmware	YES	-	-
Diagnostic Readout (Field Service & Debug)	YES	-	-
FPGA Register R/W	YES	YES	via PCIe only
High-Level DAQ Functions	-	YES	limited; via PCIe only
Set Power-On Defaults	YES	YES	-
Control Motorized VDL	YES	YES	-

FPGA Image Processing Pipeline

OCT image processing blocks implemented in the FPGA processing pipeline.

Analog-to-Digital Conversion

Two 12-bit Analog-to-Digital Converters (ADC) perform digitization of two separate analog OCT signals at the front end of the image processing pipeline. These two channels are typically used for acquiring horizontal $(\textbf{H})$ and vertical $(\textbf{V})$ polarization signals for OCT interferometers in a polarization diverse configuration, but one channel can be disabled for non-polarization diverse configurations.

Windowing and Zero-Padding

The number of raw data samples $(N_{samp})$acquired for each A-scan depends on the laser source bandwidth and k-clock determined scan depth, up to a maximum of 2048 samples. A programmable apodization window function consisting of $N_{samp}$ values concatenated with additional zeros for a total vector length of 2048 is multiplied by the sampled raw data, effectively windowing and zero-padding the raw data to 2048 samples in a single step. The window function is complex-valued in order to provide compensation of group delay dispersion.

Fourier Transformation, Complex Modulus, and Polarization Mixer

Following Inverse Fast Fourier Transformation of each channel, the negative frequencies are truncated (they are symmetric and thus redundant) and the complex modulus is calculated on the remaining 1024 samples. The complex modulus of each channel is the square root of the sum of the squares of the real and imaginary components of the IFFT output. The polarization diverse output is the square root of the sum of the squares of the complex moduli of the H and V channels. Conveniently, a level of square roots and squares cancel out, reducing the overall result to the square root of the sum of the squares of the two $Real$ and two $Imaginary$ components of the two channels, a linearly-scaled OCT intensity signal:

$$\textbf{I}=\sqrt{(Re\textbf{H})^2+(Im\textbf{H})^2+(Re\textbf{V})^2+(Im\textbf{V})^2}$$

Logarithmic Compression and Dynamic Range Reduction

OCT images are typically displayed with a logarithmic intensity scaling on a monitor which provides no more than 256 grey levels (8-bit). The logarithmically-scaled OCT intensity signal or power spectrum $(\textbf{PS})$ is calculated with 16-bit precision as:

$$\textbf{PS}_{16}=20log_{10}(\textbf{I})= 3.0103 \times2log_2(\textbf{I})$$

For dynamic-range reduction (16- to 8-bit), a user-configurable GAIN and OFFSET is applied to scale and shift the output values appropriately prior to truncation of the lowest byte. This is analogous to a Brightness/Contrast or White-level/Black-level adjustment. The result is an 8-bit power spectrum appropriate for display:

$$\textbf{PS}_8=\textbf{GAIN} \times 2log_2(\textbf{I})+\textbf{OFFSET}$$

JPEG Compression (Ethernet interface only)

JPEG compression with a quality factor of 30 results in average bandwidth reduction of approximately 3x on the transmitted OCT intensity image. Although JPEG compression is lossy, this compression ratio provides qualitatively equivalent images as the non-compressed mode and is typically sufficient for image analysis by the naked-eye. Users applying advanced quantitative image processing algorithms such as automated classification or texture analysis should investigate the effects of logarithmic, dynamic range, and JPEG compression on an individual basis and consider using the PCIe interface if access to non-compressed data is required.